FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18275952
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG487440.2667111404100865No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA354190.19380112182391374No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG233950.12800974745392196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT218200.016.55297368
ACGAGTT245500.014.66936669
GGCCACG271300.013.32612665
CCACGAG275550.013.18398867
GCCACGA275550.013.17128666
GGATAAC303600.012.2168781
ACGGCCA298250.012.21568363
AACGGCC296950.012.19844562
GGGGTAT17100.012.0843121
GCGGGGC12700.011.8585271
GCGAGGA165850.011.84713651
GCCGGGG20750.011.8153321
GAACGGC305100.011.80357461
GTGAACG312350.011.59682659
CGGCCAC312400.011.58405364
TGAACGG310350.011.57006960
GCGGGGA30450.011.5021421
GGCCATC323550.011.29261910
ACATGGC330800.011.27981956
CATGGCC329000.011.1693177
Produced by FastQC (version 0.11.2)