FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17995464
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG442320.2457952737423164No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA323410.17971751103500302No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG206350.11466778517075192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT201700.015.078547568
GCGGGGG14600.013.6748111
ACGAGTT234400.013.0795369
GCGGGGA26850.012.9148761
GGCCACG252400.012.11919465
GGGGCCC7550.012.05235870
GCCACGA258100.012.00054366
CCACGAG264050.011.70361967
GTCCGCT12450.011.5349011
CGGGGGG12550.011.4429891
GGATAAC279400.011.4331991
GCCGGGG18400.011.4217531
AACGGCC278200.011.10855762
ACGGCCA280050.011.07260563
GAACGGC282250.010.911839561
GCGCGCT17450.010.8392111
GCGGGCT13250.010.8384541
GGGCGCT6502.3646862E-1110.777451
GTGAACG289650.010.68142559
TGAACGG287950.010.67158960
Produced by FastQC (version 0.11.2)