FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15963799
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCGGTATTTAGCCTTGGA258640.16201657262159214No Hit
GTGCGATCCGTCGAGTTATCATGAATCATCGGAGCGACGGGCAGAGCCCG240940.15092898626448503No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG212550.1331449988815319No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC211590.1325436382655532No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA209860.13145993632217495No Hit
CTGCAAAGGATTCTGCCCGCCGTCCGGTCGAAATTAATCTTCAAGGCGGC191160.11974593265675669No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT161340.10106616852291862No Hit
GCATCATTCAAATTTCTGCCCTATCAACTTTCGACGGTAGGATAGGGGCC159790.10009522169503637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA43250.034.2547231
CTGCCAG50300.029.7148112
GCCGTGC42650.026.6067711
GCCAGTA55800.026.5345944
TGCCAGT59050.025.1337723
GCACACG27700.024.00640111
TAGTCAT66850.022.6165339
GCCGCAG56200.022.0614051
ACGTCTG30600.021.73169115
CCAGTAG70100.021.1716215
CAGTAGT71150.021.0068366
CGCGGGG80550.020.68184369
GTCACGT32450.020.38521629
CGTGCGA58550.020.266773
TAAATGC89500.020.06051669
GTGCGAT91600.019.882641
AGTCACG33750.019.80729128
CACACGT33900.019.71927812
GTCATAT77850.019.1965211
TGCGATC96400.018.7000712
Produced by FastQC (version 0.11.2)