FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16242038
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG335910.20681517922812395No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA244970.15082466867766225No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG198280.12207827613751428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22250.028.94366648
GTATGCC25950.025.35608547
CGTATGC26500.024.8297546
GGCCTTA32350.022.0712536
GCACACG35750.020.65704211
CTCGTAT34650.020.10096444
ATGCCGT34200.019.75123449
TCGTATG36450.018.43611545
ACGTCTG41850.017.64566415
GCCTTAT40700.017.28502537
GTCACGT41400.016.99276729
GTGGCCT45900.016.08948534
CACACGT45850.015.80134512
TATCTCG45950.015.69083641
TGCCGTC43000.014.81360150
AGCACAC49400.014.73617410
TCTCGTA48050.014.6410143
CACGAGT172950.014.52946368
TCACGTG50450.014.15272430
AGTCACG51500.013.7961728
Produced by FastQC (version 0.11.2)