FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16261922
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG428290.26336985259184004No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA289620.17809702936713137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT192500.015.85375668
ACGAGTT218200.014.08266669
GGCCACG233650.013.1066465
GGATAAC243300.013.08601951
GCCACGA240650.012.81253866
CCACGAG242950.012.59040367
GCGGGGG12800.012.3136931
ACGGCCA255850.012.092422563
AACGGCC252450.012.03346862
GAACGGC257250.011.808961
CGGGCAT12550.011.721721
GGGGTAT13450.011.7186071
TGAACGG263850.011.56663960
GGCCATC264600.011.56037910
GTGAACG264850.011.54939659
ACATGGC272800.011.5357166
CATGGCC268100.011.5008047
ATGGCCA272100.011.3831868
CATGCGC269000.011.293570528
CGGCCAC273150.011.28814364
Produced by FastQC (version 0.11.2)