FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21808299
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG632940.2902289628365789No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA421650.19334382750346554No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG244190.1119711353920817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT274000.017.76765468
GTCCGCA20250.016.24696534
ACGAGTT311400.015.65608169
CGTCCGC20650.015.59312733
GGCCACG340900.014.34246865
GCCACGA347350.014.15668466
CCACGAG351700.014.01144167
GGATAAC361100.013.8305161
TATGCCG25450.013.75237448
CGCACAT25150.013.22086937
ACGTCCG24900.013.07203132
AACGGCC372450.013.05232162
ACGGCCA375050.012.99913563
GAACGGC379100.012.80475361
GTGAACG388600.012.58183959
CGGCCAC393100.012.49131464
TGAACGG390750.012.46776960
CCGCACA26300.012.37660836
ACATGGC400800.012.3578516
CGCGGGG15050.012.3334841
Produced by FastQC (version 0.11.2)