FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22400998
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG553150.24693096262943282No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA368290.16440785361437915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT249750.016.8021268
ACGAGTT289300.014.54134369
GGCCACG307950.013.59262565
GCCACGA314650.013.38093266
GGATAAC331750.013.3339251
CCCGTCC20500.013.31712733
CCACGAG320500.013.14761467
GCGGGGG18850.013.0062551
AACGGCC330600.012.76726262
ACGGCCA333300.012.66380963
GAACGGC336400.012.53648261
TGAACGG346850.012.20931260
ACATGGC365750.011.991556
GTGAACG350250.011.98087659
CGGCCAC356600.011.91488264
CATGGCC360650.011.8972147
GGCCATC357300.011.86196710
CGTGAAC357600.011.79337258
CATGCGC358600.011.71231528
GGGCTCC362100.011.66629752
Produced by FastQC (version 0.11.2)