FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22952012
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG602050.2623081584307293No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA414340.18052447863829976No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG238370.10385581882756073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT269250.015.7153768
ACGAGTT311450.013.65328869
GCGGGGG19650.012.4767611
GGCCACG339350.012.46902665
GCCACGA346700.012.23491466
GGATAAC362450.012.2238721
CCACGAG348600.012.19840267
GCGCGCT22350.011.9097441
GCACACG38400.011.75743311
AACGGCC366850.011.59148262
ACGGCCA371200.011.43681163
GAACGGC370250.011.39023861
ACATGGC394100.011.1377686
GTGAACG380550.011.11883159
TGAACGG379750.011.09612760
GCCGGGG22450.011.0766491
CGGCCAC389250.011.00542564
CATGGCC394300.010.9617537
GCGAGGA201850.010.8794061
GGCCATC395100.010.82454710
Produced by FastQC (version 0.11.2)