FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19015982
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG472470.2484594274437155No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA316830.16661248417252394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT213800.015.97680368
GCGGGGG16150.015.613211
ACGAGTT242600.013.97903969
CGGGGGG13850.013.1487891
GGCCACG262350.012.92688665
GCCACGA272100.012.55362166
GGATAAC285150.012.4045271
CCACGAG272600.012.40220967
AACGGCC281550.012.09504162
ACGGCCA285650.012.00717263
GAACGGC284150.011.84882261
TGAACGG288400.011.72275360
GTGAACG292550.011.56842159
ACATGGC311900.011.3237966
GGCCATC305850.011.24854210
CGTGAAC301600.011.221292558
CGGCCAC305850.011.21418364
CATGGCC309950.011.1786717
GCGGGGA30500.011.1379191
GCCGGGG19800.010.9662641
Produced by FastQC (version 0.11.2)