FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20752854
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG811680.39111728921718425No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA513000.24719491593782716No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG305240.14708338429018003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT301900.020.34534568
ACGAGTT332950.018.46879469
TATGCCG24250.018.04105248
GCCACGA384300.016.11954966
GGCCACG384300.016.02863165
CCACGAG385300.015.98687767
CGTATGC28950.014.99113846
GTATGCC30150.014.97493647
GGATAAC428650.014.7873221
ACGGCCA426350.014.54623563
AACGGCC432100.014.28786862
CGGCCAC434850.014.16535164
GAACGGC442050.013.90265861
GCGCGCT17900.013.6948971
TGAACGG451150.013.58347860
GTGAACG452400.013.545979559
ACATGGC477650.013.205786
CGTGAAC468850.013.12308758
CATGGCC476350.013.0926447
CGAGTTC484350.013.0209470
Produced by FastQC (version 0.11.2)