FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n02_QG821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n02_QG821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40097428
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA333200.06.921499370
AGTGTAG364100.06.795487469
GAGTGTA395100.06.271132570
TAGATCT233500.06.264858769
TGTAGAT374050.05.697802570
AGAGTGT527100.05.63018470
GTAGATC359800.05.22315570
ATCTCGG210850.05.21165770
AAGAGTG604100.05.173237370
AGATCTC282400.05.14284270
CTCGGTG154500.05.14182370
GTGTAGG933400.05.125313370
GATCTCG243500.05.116473770
Produced by FastQC (version 0.11.5)