FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n02_QG709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n02_QG709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41422219
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG320650.06.362809769
GTGTAGA305750.06.20360270
GAGTGTA343200.05.86316668
TAGATCT223900.05.67366470
AGAGTGT467200.05.228338270
ATCTCGG215850.05.123256770
GTAGATC328000.05.089277769
TGTAGAT345900.05.068725669
Produced by FastQC (version 0.11.5)