FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n02_QG705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n02_QG705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44206364
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA365450.06.57871370
AGTGTAG397700.06.51160970
GAGTGTA422550.06.36063869
TAGATCT254200.06.16757870
GATCTCG263250.05.862493570
TGTAGAT409950.05.74508470
AGAGTGT570600.05.341934770
ATCTCGG239350.05.33668570
CTCGGTG173300.05.310915570
GTAGATC394600.05.064034569
Produced by FastQC (version 0.11.5)