FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n02_QG1119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n02_QG1119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48593494
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT334050.06.58953870
GTGTAGA467050.06.35401670
CTCGGTG220000.06.203799270
AGTGTAG513000.06.119159769
GAGTGTA552300.06.082930669
ATCTCGG296050.06.028661370
GATCTCG332650.05.98605370
GTAGATA99450.05.87661870
TGTAGAT518200.05.747089470
GTAGATC492250.05.46710469
AGATCTC387600.05.272843470
AGAGTGT726250.05.006626670
Produced by FastQC (version 0.11.5)