FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16313401
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5007643.06964807644954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27350.020.4143771
GAGGGGG22100.011.4118492
GGAGGGG23500.011.3564371
ATGCCGT12500.09.8000944
CGGGGGG77800.06.3640831
CAGTCAC27700.05.813274469
GCCACGG22105.456968E-125.5612461
CGCAGGC9707.0581195E-45.413352570
GAACTCC68950.05.22932669
AGCGGTT13401.4791538E-55.2232911
CCAGTCA37800.05.093359568
CCACGGT17951.5818841E-75.0736992
Produced by FastQC (version 0.11.5)