FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40097428
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58800.017.3799648
GTATGCC61650.017.03063447
CGTATGC63750.016.24998946
TCGTATG83850.012.06245845
ATGCCGT90000.011.31601949
GGCCTTA130350.010.09551936
CTCGTAT112800.09.24589144
GCCTTAT155950.08.37097137
TGCCGTC126250.08.15001850
GCCGTCT137550.06.94624551
GTCACGT411550.06.83046770
AGTCACG426700.06.719084369
GTGGCCT226050.06.53529870
CTGCTTG124900.06.529509559
TGGCCTT215550.06.413642435
TCTCGTA163500.06.400229543
CAGTCAC510250.06.30507170
CACGTGG420850.05.864341770
TATCTCG202050.05.820037441
ATCTCGT198400.05.80358542
Produced by FastQC (version 0.11.5)