FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG722.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG722.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42172658
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC58350.013.25545947
TATGCCG60100.012.927731548
CGTATGC61950.012.54165846
TCGTATG83250.09.54298945
ATGCCGT84350.09.12811149
CGTCCGC225200.07.74152270
CACGTCC279500.07.69045370
ACGTCCG239100.07.56965970
AGTCACG398850.06.62678370
GTCACGT390900.06.58244270
TCCGCAC196050.06.464085670
TCACGTC371650.06.405310670
GTCCGCA223800.06.28827270
CCGCACA162400.06.22984470
CTCGTAT127800.06.10682944
CAGTCAC479500.06.05245170
TGCCGTC127800.06.052088750
CGCACAT149000.05.590352537
GCCGTCT146650.05.321943851
CCAGTCA544850.05.30711169
Produced by FastQC (version 0.11.5)