FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37819636
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC51650.014.50053747
TATGCCG50800.014.0542748
CGTATGC52250.013.59722946
ATGCCGT75500.09.68815649
TCGTATG67200.09.63479745
CGTATCT100950.08.66731639
CCGTATC147300.07.555701738
TCCGTAT162700.07.07717437
GTATCTC129100.06.72315340
CTCGTAT110200.06.446944744
TGCCGTC109100.06.319516750
TTCCGTA203000.06.29288136
CAGTCAC378700.05.53718270
GTCACAG315900.05.474375270
GTATCGC39550.05.30948440
GTTCCGT252750.05.28963335
TCTCGTA159000.05.084571443