FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44206364
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG76500.020.17536748
CGTATGC77200.020.08305446
GTATGCC78200.019.8709947
TCGTATG96900.016.25292645
ATGCCGT108750.014.51418249
TGCCGTC146950.010.66976150
CTCGTAT146400.09.99258944
CTGCTTG147600.09.24817459
GCCGTCT164100.09.08549851
TCTCGTA206350.07.360860343
TGCTTGA184850.07.23321660
CCGTCTT214400.07.117308652
ATCTCGT220700.06.739557742
GTCACAG450600.06.487100670
AATCTCG255500.06.191491641
CACAGTC376550.06.11728370
AGTCACA530200.06.0215870
CAATCTC266650.05.788248540
AGTCAAC345000.05.69244870
CAGTCAC557800.05.692103469
Produced by FastQC (version 0.11.5)