FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG1119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG1119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48593494
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC81500.011.07903247
TATGCCG83350.010.833137548
CGTATGC87400.010.6514846
TCGTATG111800.08.29551545
ATGCCGT124900.07.64962149
GATATAT225800.07.02341870
CTCGTAT141150.06.942508744
CACACTG495900.06.58660870
GTCACAC536100.06.471457570
TGATATA280400.06.354977670
TGCCGTC164400.06.0245450
CAGTCAC686350.05.8708970
AGTCACA641750.05.85339970
ATATATC199200.05.72931370
TCACACT595550.05.660858670
CTGCTTG166350.05.55471759
ACACTGA577450.05.38966370
GCCGTCT183250.05.366657351
TATATCT177700.05.317670339
CCAGTCA767100.05.28464869
Produced by FastQC (version 0.11.5)