FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG1118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG1118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40768614
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58850.014.62957746
GTATGCC59100.014.62692947
TATGCCG59800.014.57276148
TCGTATG78750.011.24379945
ATGCCGT90800.09.40474649
CTCGTAT114350.07.74332544
TGCCGTC121350.07.065957550
AGTCACG292350.06.753507670
GCCGTCT126350.06.45397951
ACGAGTG230700.06.175899570
CACGAGT236350.06.087389569
CTGCTTG125650.05.7661659
GTGGATA175750.05.735224236
CAGTCAC356700.05.505669
GTCACGA292950.05.30559869
TCTCGTA170750.05.24715643
TCACGAG291650.05.245330370
GGATATC180700.05.093892638