FastQCFastQC Report
Wed 12 Feb 2020
H5YFHBGXF_n01_QG1112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YFHBGXF_n01_QG1112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46091300
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC68550.014.19323147
TATGCCG67250.014.10333848
CGTATGC68550.013.68265346
TCGTATG91650.010.19581145
GTACGTA149600.08.81989935
ATGCCGT101700.08.74091249
TACGTAA157750.08.36419136
CCGTACG200750.08.318027570
ACGTAAT153700.08.19744937
CTCGTAT120950.07.725871644
CGTACGT176900.07.63865670
CGTAATC175150.06.614042838
CACCGTA275150.06.60321470
ACCGTAC278500.06.54892670
TGCCGTC137850.06.524853750
GTCACCG363000.06.364947370
TAATCTC180750.05.71204340
TCACCGT350300.05.53639670
AGTCACC424650.05.51508970
TCTCGTA180500.05.487200743
Produced by FastQC (version 0.11.5)