FastQCFastQC Report
Sat 8 Feb 2020
H5YC7BGXF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YC7BGXF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438841328
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25866810.5894342294032981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12630850.069.205021
AGCAGTG13682050.063.744862
GCAGTGG15208200.057.3497283
CAGTGGT15715250.055.4566354
AGTGGTA16699950.052.347375
TGGTATC17321100.050.2869767
GTGGTAT17526700.049.837526
GGTATCA17809250.048.9530648
GTATCAA18460150.047.0667769
CATGGGG10794000.028.81886724-25
AGAGTAC17600050.028.0546218-19
GCAGAGT17836650.027.77716-17
TACATGG17485950.027.77416622-23
CAACGCA17847250.027.69846312-13
ATCAACG18142350.027.25738710-11
ACGCAGA18518100.026.73762714-15
ACATGGG16907400.025.17982324-25
AGTACAT19777450.024.8566220-21
CAGAGTA17612300.024.7944318-19
CATGGGA5368900.024.5974424-25
Produced by FastQC (version 0.11.5)