FastQCFastQC Report
Sat 8 Feb 2020
H5YC7BGXF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YC7BGXF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438841328
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG25113110.572259456839489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGAG440950.016.9999283
GCCCGAA726400.016.885871
TCGGGAC610750.016.4472161
GGGCTAC629850.016.402731
CCGGACA815050.016.3980981
CTGGACG536100.016.3808821
CCCGGAA605950.016.2669471
AGGGAAG421100.016.2468663
GGGACCT631150.016.1754061
TGCGACG615950.016.0332221
GCGTGGT503150.015.8997633
AGGGCCT801800.015.5985271
CTGGCAG689050.015.5141351
GAGACCC848300.015.3402311
GTGCTGG524750.015.2525111
GACAGCC1231700.015.2209911
CTCGAGG768900.015.2125761
CGGGACG250900.015.193432
TACCGGG562500.015.1444931
TCGGGTG432600.015.1308971
Produced by FastQC (version 0.11.5)