FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23246745
Sequences flagged as poor quality0
Sequence length99
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3760651.6177103504168004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG243750.054.262492
TGACCCG42550.044.5994389
GTATCAA313950.042.2233969
ACGAGGT48700.040.571592
GCAGTGG366450.036.7446823
GGTATCA364150.036.6282468
AGTGGTA371850.035.9682855
AAGCAGT383250.035.103271
TCAGGTC44350.034.5041289
GTGGTAT397100.033.6201866
CTGAACT71550.033.5435379
CAGTGGT400000.033.4456864
TGCATCC52700.032.4711344
TGACTGA77600.031.040946
TTGACTG79050.030.6502825
AACGAGG69850.028.6176051
CGTGCAT37600.028.20463224-25
TGGTATC483200.027.7375147
AATTCTA78900.027.2354019
CGTTAAA78300.027.1403064
Produced by FastQC (version 0.11.5)