FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_ICAL2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_ICAL2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83117684
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5062990.6091351149774578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1892250.057.338362
AGTGGTA1978800.054.7628945
GCAGTGG2073450.052.170313
AAGCAGT2132600.051.1608431
GTGGTAT2313600.046.907366
GGTATCA2359550.045.871068
CAGTGGT2396600.045.2557034
GTATCAA2456850.044.041739
TGGTATC2501600.043.3700877
ACGCAGA2107550.028.75664114-15
CAACGCA2212500.027.43143812-13
AGTACAT2250950.026.83358820-21
TACATGG2286950.026.28248822-23
GCAGAGT2338500.026.02823816-17
GTACATG2118200.025.32600622-23
AGAGTAC2428850.025.0855918-19
CATGGGG1550800.024.94349324-25
ATCAACG2470350.024.80082710-11
TATCAAC2241000.024.25440810-11
TCAACGC2278150.023.73108912-13
Produced by FastQC (version 0.11.5)