FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_ICAL2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_ICAL2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532266
Sequences flagged as poor quality0
Sequence length99
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170390.6728755983771058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG58850.059.1592832
AGTGGTA64400.054.510155
AAGCAGT66700.052.1874731
GCAGTGG67000.051.828383
GTGGTAT74300.047.4413266
GGTATCA76150.046.1712048
CAGTGGT78150.044.9735954
GTATCAA77950.044.877039
TGGTATC79450.044.251717
ACGCAGA67900.028.5026114-15
CAACGCA69850.027.80653212-13
AGTACAT72500.026.79015520-21
GCAGAGT73700.026.25926816-17
TACATGG72850.026.24583822-23
GTACATG67950.025.43416822-23
ATCAACG78050.025.24228710-11
AGAGTAC77350.025.1103618-19
TATCAAC71650.024.34799810-11
CATGGGG51000.024.21775824-25
AACGCAG73800.023.4817814-15
Produced by FastQC (version 0.11.5)