FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_ICAL2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_ICAL2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62595332
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3968790.6340392922590458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1431950.057.3370672
AGTGGTA1497850.054.7246675
GCAGTGG1571900.052.1397443
AAGCAGT1603600.051.511531
GTGGTAT1744700.047.0777976
GGTATCA1786150.045.9258238
CAGTGGT1812900.045.297574
GTATCAA1853000.044.193859
TGGTATC1887000.043.5306177
ACGCAGA1591850.028.95760714-15
CAACGCA1665200.027.73836312-13
AGTACAT1692000.027.09614620-21
TACATGG1728650.026.40826822-23
GCAGAGT1761650.026.23858616-17
GTACATG1597950.025.40403622-23
AGAGTAC1830550.025.27996618-19
ATCAACG1854750.025.11830110-11
CATGGGG1163800.025.01793524-25
TATCAAC1690700.024.27876310-11
TCAACGC1716400.023.83485612-13
Produced by FastQC (version 0.11.5)