FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_ICAL2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_ICAL2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65476276
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4058560.6198519903606002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1501800.057.342582
AGTGGTA1574200.054.5561265
GCAGTGG1645100.052.2773323
AAGCAGT1683700.051.3497921
GTGGTAT1822900.047.1381036
GGTATCA1874150.045.8675168
CAGTGGT1893600.045.469114
GTATCAA1944500.044.1569189
TGGTATC1979950.043.481987
ACGCAGA1665500.028.87073914-15
CAACGCA1747550.027.5993712-13
AGTACAT1778750.026.9942920-21
TACATGG1816900.026.31168422-23
GCAGAGT1837600.026.26749816-17
GTACATG1678400.025.42591522-23
AGAGTAC1922800.025.12904718-19
CATGGGG1233600.025.0960724-25
ATCAACG1955300.024.8921910-11
TATCAAC1762900.024.42659210-11
TCAACGC1806700.023.77979712-13
Produced by FastQC (version 0.11.5)