FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_CTR2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_CTR2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65231682
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2677150.4104064034405859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1416450.055.980842
AGTGGTA1491500.052.9828765
AAGCAGT1575700.050.6145481
GCAGTGG1566250.050.498873
GTGGTAT1730000.045.7614066
CAGTGGT1756500.045.0507164
GGTATCA1759950.044.9056748
GTATCAA1847350.042.6202779
TGGTATC1868650.042.3612677
ACGCAGA1585550.028.39233614-15
CAACGCA1657300.027.18603712-13
AGTACAT1711050.026.27013420-21
GCAGAGT1740150.026.0286216-17
TACATGG1725800.025.87119922-23
CATGGGG1147650.024.91826824-25
AGAGTAC1828250.024.77276418-19
GTACATG1610800.024.39007422-23
ATCAACG1865700.024.36167710-11
TCAACGC1692350.023.2942612-13
TATCAAC1698050.023.25361610-11
Produced by FastQC (version 0.11.5)