FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_CTR2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_CTR2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71739878
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2855260.3980017919740538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1559800.055.493322
AGTGGTA1639450.052.6737945
AAGCAGT1730150.050.321041
GCAGTGG1724600.050.11873
GTGGTAT1902950.045.4512756
CAGTGGT1931500.044.760044
GGTATCA1937250.044.545838
GTATCAA2038150.042.339189
TGGTATC2046050.042.3113067
ACGCAGA1746600.028.19822114-15
CAACGCA1826850.026.98780312-13
AGTACAT1880800.026.1168820-21
TACATGG1899350.025.6838822-23
GCAGAGT1928500.025.64914916-17
AGAGTAC2007950.024.61907618-19
CATGGGG1269550.024.5428524-25
GTACATG1774700.024.12230722-23
ATCAACG2063300.024.09140810-11
TCAACGC1864200.023.13574812-13
TATCAAC1871900.023.10306210-11
Produced by FastQC (version 0.11.5)