FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_CTR2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_CTR2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41558778
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1704850.41022621021243694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG905150.056.1978072
AGTGGTA943250.053.867365
AAGCAGT1005900.050.763681
GCAGTGG1002500.050.723523
GTGGTAT1103850.046.0542146
GGTATCA1120400.045.3340578
CAGTGGT1122250.045.3283964
TGGTATC1185200.042.97437
GTATCAA1181800.042.886099
ACGCAGA1007100.028.62827114-15
CAACGCA1047600.027.5406112-13
AGTACAT1088400.026.4127720-21
GCAGAGT1104750.026.24439816-17
TACATGG1101750.025.95087622-23
CATGGGG719250.025.29008124-25
AGAGTAC1160000.025.0160318-19
ATCAACG1186650.024.62994210-11
GTACATG1029550.024.3776322-23
TCAACGC1075300.023.4891612-13
TATCAAC1084350.023.46373210-11
Produced by FastQC (version 0.11.5)