FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n02_CTR2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n02_CTR2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70812650
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2825500.3990106287506541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1551150.055.320262
AGTGGTA1617450.053.0847325
AAGCAGT1709850.050.448331
GCAGTGG1703150.050.29913
GTGGTAT1880600.045.693726
GGTATCA1907750.044.9761248
CAGTGGT1909000.044.9630364
GTATCAA2011450.042.5729649
TGGTATC2027000.042.4195567
ACGCAGA1726500.028.30609514-15
CAACGCA1796800.027.2455112-13
AGTACAT1857750.026.23897220-21
GCAGAGT1890800.025.96144716-17
TACATGG1878900.025.83568622-23
CATGGGG1253000.024.91327924-25
AGAGTAC1975700.024.8820618-19
ATCAACG2031350.024.31839610-11
GTACATG1749800.024.31116122-23
TCAACGC1839650.023.24770712-13
TATCAAC1853800.023.19974110-11
Produced by FastQC (version 0.11.5)