FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23246745
Sequences flagged as poor quality0
Sequence length27
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGC1140270.49050738071071887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG594580.2557691410130752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC120500.020.32339921
GGGAATG57100.019.7695961
CGGAAGA41350.018.6152174
GAGGAGG25700.018.3056244
CCGCAGG39000.018.2537615
ACGAGGA39650.018.0763261
GCGGAGT24950.018.0635493
AGGACGA22050.017.8502628
TGCCAAA48900.017.7823661
TGCGGAG25550.017.768562
ACTGGAG26000.017.60105913
GACTAAC37350.017.5244921
AGGTCCG70900.017.437981
GCACGCC36000.017.4349489
CCGGGAT32050.017.4297961
TTGCACG33100.017.3836045
CCTACCA40200.017.304631
CTCGGGA34150.017.1913091
GGCACCA25250.017.1736986
AAACCGG28300.017.1559562
Produced by FastQC (version 0.11.5)