FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_ICAL2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_ICAL2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83117684
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA553500.019.7377918
CGCAGGA507000.019.6470626
AGGACGA251350.019.616588
GGGAATG513550.019.595681
GAGGAGG308700.019.4314544
CCGCAGG512000.019.3936865
ATAGACC414700.019.3849261
TGAGGAG363950.019.375283
CTGCGGA393400.019.33751
GAATGAA368000.019.2056081
TGCCAAA403000.019.163461
TGCGGAG295150.019.0856082
TGACGGC251850.019.0408061
AATGGAC354100.019.0274319
TACACGA494300.019.0184121
AGATTGC750150.019.0071051
ATGGACG354250.019.00456810
GCGGAGT296750.018.9933173
GATGAAA353600.018.9810731
ACGAGGA243850.018.9679091
Produced by FastQC (version 0.11.5)