FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_ICAL2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_ICAL2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532266
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGA15300.020.038336
CCGCAGG15500.019.7797725
GGGAATG15500.019.712811
GAATGAG4700.019.6587073
AGATATG10800.019.6376328
AGGAGAG5250.019.5991335
ATGGACG10200.019.4546410
ATAGACC12050.019.4314421
GCACGCC9850.019.3997769
TGAGGAG11700.019.3837593
GAGGAGG9600.019.358524
GAATGAA13150.019.3231051
CAATGGA15950.019.2213428
CTGCGGA12200.019.1925321
AGGACGA6950.019.1858238
GGATTGG16350.019.1363910
TACACGA14350.019.0974711
AGTGATA9300.019.0794267
CTCGAAA4850.019.0514561
AGATTGC21350.019.0326981
Produced by FastQC (version 0.11.5)