FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_ICAL2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_ICAL2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62595332
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA403750.019.7250618
GGGAATG376350.019.6386831
AGGACGA188650.019.6023358
CGCAGGA383000.019.5272876
CTGCGGA289200.019.4099331
GAGGAGG233950.019.3837684
GAATGAA285500.019.3819661
TGAGGAG278800.019.3650263
CCGCAGG385650.019.3495435
ATAGACC315050.019.3437771
TACACGA376750.019.208141
GATGAAA264900.019.1847761
AATGGAC259400.019.1657289
ATGGACG260450.019.04413210
TGCGGAG216150.019.032172
TGCCAAA291750.019.020741
TGACGGC186150.019.0146831
AGATTGC564950.018.9687561
GCGGAGT217350.018.94643
GCACGCC273600.018.9193469
Produced by FastQC (version 0.11.5)