FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_ICAL2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_ICAL2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65476276
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA430400.019.7476448
AGGACGA192500.019.7227928
CGCAGGA397000.019.6372346
TGAGGAG297750.019.550063
ATAGACC334350.019.5147131
GGGAATG393700.019.5039441
CCGCAGG400500.019.4446645
GAGGAGG249400.019.4163634
CTGCGGA303700.019.3993761
TGCGGAG224800.019.2666262
AATGGAC272300.019.1945519
GATGAAA274250.019.1355921
TACACGA379500.019.0689051
GAATGAA294700.019.061891
TGCCAAA312100.019.0286461
GCGGAGT228100.019.015453
AGATTGC583450.018.9683341
GTGATAA286750.018.966938
ATGGACG275750.018.96201710
TGACGGC203650.018.9222981
Produced by FastQC (version 0.11.5)