FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_CTR2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_CTR2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65231682
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG292150.019.2897261
CTGCGGA233900.019.1285321
ACGCCGA230300.018.7937931
GCCGAAG165500.018.7853853
CTAGAGA226500.018.7511984
TGACGGA165850.018.72657810
TCTGGAA293800.018.6167091
CAATGGA198200.018.5836778
ATAGACC213100.018.5368391
TATTGAG237600.018.2994693
CACCGAA152550.018.266888
CGCCGAA135050.018.2473682
CTGAAAC223200.018.2437441
ATGACGG171200.018.1597719
CAATGAA188100.018.1357988
AGTGAGG240400.018.1308691
GGTATTG375750.018.1222171
GTGCGAA169500.018.0135978
TAGAGAA173100.018.0029855
GGAAAGC166200.017.986921
Produced by FastQC (version 0.11.5)