FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_CTR2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_CTR2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71739878
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG311400.019.273841
CTGCGGA255050.019.172361
ACGCCGA253050.018.9545921
CTAGAGA263850.018.8385854
TCTGGAA321300.018.778011
GCCGAAG180150.018.7555623
TGACGGA184950.018.6433710
CAATGGA216650.018.5276748
ATAGACC236650.018.3646891
CGCCGAA146950.018.277212
CACCGAA167600.018.2177898
ATGACGG189750.018.2049479
CTCGGGA243850.018.1970671
CTGAAAC249700.018.1744331
CAATGAA201800.018.1636878
GCGCGAT205300.018.1258951
AGTGAGG273350.018.1077791
TATTGAG253650.018.0356163
GGTATTG403200.017.9872021
GGGACCT184650.017.9864121
Produced by FastQC (version 0.11.5)