FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_CTR2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_CTR2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41558778
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG185400.019.1540031
CTGCGGA143700.019.027421
TGACGGA102450.018.8983910
ACGCCGA147450.018.8853241
GCCGAAG105500.018.87963
CAATGGA127350.018.7238038
TCTGGAA181500.018.715151
CTAGAGA149050.018.6958984
ATAGACC138050.018.47511
CGCCGAA86650.018.309412
TATTGAG149450.018.3016283
TAGAGAA111550.018.232115
CTGAAAC143550.018.09641
ATGACGG107150.018.0890339
GGGACCT107550.018.051871
GCGCGAT118700.018.0280671
CAATGAA122100.018.0068138
GGTATTG238650.017.9644371
AGTGAGG155900.017.962741
TTGGCGC124650.017.95852910
Produced by FastQC (version 0.11.5)