FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_n01_CTR2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_n01_CTR2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70812650
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA247150.019.2625751
GGGAATG321400.019.1935561
TCTGGAA315900.018.8629531
ACGCCGA241250.018.8588331
GCCGAAG181300.018.7698143
ATAGACC236000.018.7133141
CTAGAGA253800.018.6371884
TGACGGA182900.018.50208910
CAATGGA212550.018.4996818
ATGACGG185250.018.3183639
TATTGAG256250.018.205023
GCGCGAT196700.018.203021
AGTGAGG271000.018.1833841
GGGACCT181000.018.1692351
CACCGAA165100.018.124728
CAATGAA204150.018.0831058
CGCCGAA145150.018.0770782
CTGAAAC239200.018.0766771
GGTATTG406450.018.0732441
AAACCTG264500.017.9990371
Produced by FastQC (version 0.11.5)