FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486311291
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27084140.556930108373733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG10500400.056.549912
AGTGGTA11156800.053.17335
GCAGTGG11661750.050.8503763
AAGCAGT11840400.050.4304241
GTGGTAT12930850.045.9564556
GGTATCA13144300.045.132168
CAGTGGT13247200.044.844274
GTATCAA13664100.043.3202829
TGGTATC13992750.042.4752437
ACGCAGA11977950.027.98475814-15
CAACGCA12337200.027.2138912-13
AGTACAT12558950.026.59393520-21
TACATGG12780600.025.9907122-23
GCAGAGT13017650.025.87165316-17
AGAGTAC13512750.024.92834718-19
CATGGGG8566800.024.82709324-25
GTACATG11846350.024.81626122-23
ATCAACG13780600.024.54444310-11
TATCAAC12720900.023.36349910-11
CGCAGAG12787450.023.31140116-17
Produced by FastQC (version 0.11.5)