FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486311291
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAA8224175916.91134064168788No Hit
CTGCGA7106291014.612638307013933No Hit
GCATCT7004594914.403521015513498No Hit
AACGTC6459804513.283270653076407No Hit
GAAACC6413293413.187630060598368No Hit
TTTCTG6188431212.725246800819189No Hit
TGTAAG410476968.440621626447081No Hit
GGGGGG148168773.0467886052022592No Hit
CCGTGT24314050.49996885636776217No Hit
TTCTGT8945460.18394514307092247No Hit
GAACCC8153680.1676638019905649No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)