FastQCFastQC Report
Wed 3 Apr 2019
H5Y5CBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5Y5CBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486311291
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG2493050.019.4833531
CTGCGGA1927750.019.1234861
CAATGGA2260050.019.1062628
ATAGACC1973450.018.8174041
AGGACGA777450.018.5261468
GATGAAA1604000.018.2679161
GAATGAA1507800.018.1824971
ACGAGGA1596650.018.176391
CCGCAGG1492500.018.1368375
TGCCAAA1859700.018.0155931
CGCAGGA1498000.017.9875076
TGACGGA922250.017.94101110
GAGGAGG947600.017.8892614
CCTACCA1619550.017.838971
CGCTGGA1395550.017.7749371
TCTGGAA1755500.017.766421
TGCGGAG953100.017.6821162
CTAGAGA1086400.017.6564924
GACTAAC1524100.017.6214561
AGGTCCG2921500.017.616751
Produced by FastQC (version 0.11.5)