FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33324537
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450155013.508214682772637No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCCATATAAGAAA634110.19028321383729951No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT533830.16019127287499899No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCCATATAAGAAA440170.1320858561365759No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAA418600.12561314805363988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC759350.042.6172451
ATAGCTC775600.042.373643
CATAGCT767500.042.344842
TTGTCCG615850.042.14263512
GTCCGAC603050.042.10164314
TGTCCGA603950.042.07011413
TAGCTCT775900.042.000664
TCCGACA609700.041.82590515
CGACAAT623600.041.8134317
CCGACAA628250.041.76753616
GTTGTCC626600.041.3629911
AACCGGT152600.041.31566613
CTAACGG80350.041.3134086
TCTAACG84300.041.085925
CAATACG221800.041.0638220
GACAATA652850.040.88109218
TAACGGC81050.040.688777
TAAACCG251100.040.62974511
ACCGGTG155050.040.35860414
CGGTGTA154850.040.03561416