FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37243303
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3716490.9978948430003644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG987770.2652208371529238No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT672420.18054789608751942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT523400.033.8290941
AGCAGTG559050.031.6375482
ATCAACG566150.031.03816811
TCAACGC577800.030.44179512
AACGCAG583250.030.1962714
CAACGCA584600.030.1218113
GTATCAA587850.029.9074929
ACGCAGA589300.029.90458915
AGAGTAC585650.029.81169719
TATCAAC591700.029.7487910
GGTATCA598900.029.3399438
TGGTATC598600.029.3079177
AGTGGTA615050.028.7200375
CGCAGAG616850.028.60678516
GTACTTT607500.028.41912522
GCAGAGT624150.028.19641317
CAGTGGT629250.028.1533244
CAGAGTA632950.027.78438818
GAGTACT628650.027.5980920
GTGGTAT649600.027.1492376
Produced by FastQC (version 0.11.5)