FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36924392
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3742751.0136253563768904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG946900.2564429496902752No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT678560.18377012138750992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT527300.033.5867081
AGCAGTG559450.031.749542
ATCAACG568450.030.9471711
TCAACGC582200.030.23441712
AACGCAG587050.030.06321114
CAACGCA587550.030.01843513
GTATCAA589600.029.8376069
ACGCAGA595000.029.6832915
AGAGTAC591550.029.60544419
TATCAAC597400.029.53016910
GGTATCA603700.029.1568688
TGGTATC604600.029.1418347
AGTGGTA616050.028.7085845
CGCAGAG620550.028.5818716
CAGTGGT628800.028.2147944
GTACTTT615750.028.10127822
GCAGAGT630900.028.01050817
CAGAGTA640900.027.51698718
GAGTACT634000.027.45674720
GTGGTAT654500.026.9821416
Produced by FastQC (version 0.11.5)