FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35191359
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3514850.9987821158029163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG686670.19512460430982503No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT636460.18085689728549556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT486150.033.7618561
AGCAGTG519800.031.6809252
ATCAACG528700.030.8541511
TCAACGC541100.030.16268212
AACGCAG546800.029.92107414
CAACGCA546350.029.90513213
GTATCAA550350.029.6057789
ACGCAGA552500.029.58907915
TATCAAC553400.029.51972610
AGAGTAC552900.029.34583519
GGTATCA562550.028.9964688
TGGTATC563800.028.9244697
AGTGGTA574500.028.4684525
CGCAGAG576800.028.39037716
GTACTTT570600.028.03734822
CAGTGGT586300.028.0268694
GCAGAGT587850.027.8018517
CAGAGTA597400.027.33604818
GAGTACT591550.027.22465120
GTGGTAT611500.026.7035316
Produced by FastQC (version 0.11.5)