FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38468738
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3881101.0088971465609295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG806830.20973653983658108No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT700780.18216870020534595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT539850.033.6035961
AGCAGTG574500.031.590652
ATCAACG585300.030.82015211
TCAACGC598700.030.12655312
AACGCAG603000.029.96304114
CAACGCA603950.029.90104513
GTATCAA604100.029.754359
ACGCAGA611250.029.5727415
TATCAAC611950.029.50560410
AGAGTAC609350.029.4075919
GGTATCA619450.029.0571758
TGGTATC623200.028.8615557
CGCAGAG637850.028.42001316
AGTGGTA636750.028.386045
CAGTGGT645100.028.1307644
GTACTTT628700.028.12144322
GCAGAGT649600.027.79053917
GAGTACT651350.027.33294720
CAGAGTA661400.027.27211218
GTGGTAT673750.026.75686
Produced by FastQC (version 0.11.5)